Tenorio Elizabeth, Saeki Takashi, Fujita Katsutoshi, Kitakawa Madoka, Baba Tomoya, Mori Hirotada, Isono Katsumi
Graduate School of Science and Technology, Kobe University, Kobe, Japan.
FEMS Microbiol Lett. 2003 Aug 8;225(1):107-14. doi: 10.1016/S0378-1097(03)00507-X.
To understand the nature and function of bacterial biofilm and the process of its formation, we have performed systematic screening of a complete set of Escherichia coli genes/open reading frames (ORFs) to identify those that affect biofilm development upon over-expression. In contrast to the biofilm of strain AG1 used as a control, some of the genes/ORFs when over-expressed led to the formation of an abnormal biofilm such as thin, mat-like, filamentous or one easily detaching from various surfaces. Disruptants of selected genes were constructed in order to clarify their roles in the different stages of biofilm formation. Our results suggest that diverse metabolic pathways contribute to the development of biofilm.
为了解细菌生物膜的性质、功能及其形成过程，我们对大肠杆菌的全套基因/开放阅读框（ORF）进行了系统筛选，以鉴定那些过表达时会影响生物膜形成的基因。与用作对照的AG1菌株的生物膜相比，某些基因/ORF过表达时会导致形成异常生物膜，如薄的、垫状的、丝状的或容易从各种表面脱落的生物膜。构建了所选基因的缺失突变体，以阐明它们在生物膜形成不同阶段的作用。我们的结果表明，多种代谢途径有助于生物膜的形成。
