Gevers Dirk, Huys Geert, Swings Jean
Laboratory of Microbiology, Faculty of Sciences, Ghent University, B-9000 Ghent, Belgium.
FEMS Microbiol Lett. 2003 Aug 8;225(1):125-30. doi: 10.1016/S0378-1097(03)00505-6.
The ability of 14 Lactobacillus strains, isolated from fermented dry sausages, to transfer tetracycline resistance encoded by tet(M) through conjugation was examined using filter mating experiments. Seven out of 14 tetracycline-resistant Lactobacillus isolates were able to transfer in vitro this resistance to Enterococcus faecalis at frequencies ranging from 10(-4) to 10(-6) transconjugants per recipient. Two of these strains could also transfer their resistance to Lactococcus lactis subsp. lactis, whereas no conjugal transfer to a Staphylococcus aureus recipient was found. These data suggest that meat lactobacilli might be reservoir organisms for acquired resistance genes that can be spread to other lactic acid bacteria. In order to assess the risk of this potential hazard, the magnitude of transfer along the food chain merits further research.
利用滤膜交配实验，检测了从发酵干香肠中分离出的14株乳酸杆菌通过接合转移tet(M)编码的四环素抗性的能力。14株耐四环素乳酸杆菌分离株中有7株能够在体外将这种抗性转移给粪肠球菌，转移频率为每受体10^(-4)至10^(-6)个接合子。其中两株菌株还能将其抗性转移给乳酸乳球菌乳酸亚种，而未发现向金黄色葡萄球菌受体的接合转移。这些数据表明，肉类乳酸杆菌可能是获得性抗性基因的储存菌，这些基因可传播给其他乳酸菌。为了评估这种潜在危害的风险，沿食物链的转移程度值得进一步研究。
