Descamps Frédéric, Brouta Frédéric, Vermout Sandy, Monod Michel, Losson Bertrand, Mignon Bernard
Department of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liège, B-43 Sart-Tilman, 4000 Liège, Belgium.
FEMS Immunol Med Microbiol. 2003 Aug 18;38(1):29-34. doi: 10.1016/S0928-8244(03)00101-9.
A secreted 31.5-kDa keratinolytic subtilase (SUB3; AJ431180) is thought to be a Microsporum canis virulence factor and represents a candidate for vaccination trials. In this study, the recombinant keratinase (r-SUB3) was produced by the Pichia pastoris expression system and purified to homogeneity. Recombinant SUB3 displayed identical biochemical properties with the native protease. Experimentally cutaneously infected guinea pigs showed specific lymphoproliferative response towards r-SUB3, while no specific humoral immune response was induced except for one animal. The heterologous expression of SUB3 provides a valuable tool for addressing further investigations on the role of this keratinase in the specific cellular immune response and on its use in vaccination trials in the cat.
一种分泌型31.5 kDa角蛋白分解枯草杆菌蛋白酶（SUB3；AJ431180）被认为是犬小孢子菌的毒力因子，是疫苗试验的一个候选对象。在本研究中，重组角蛋白酶（r-SUB3）由毕赤酵母表达系统产生并纯化至同质。重组SUB3与天然蛋白酶表现出相同的生化特性。实验性皮肤感染的豚鼠对r-SUB3表现出特异性淋巴细胞增殖反应，而除一只动物外未诱导出特异性体液免疫反应。SUB3的异源表达为进一步研究这种角蛋白酶在特异性细胞免疫反应中的作用及其在猫疫苗试验中的应用提供了有价值的工具。
