Brouta Frédéric, Descamps Frédéric, Vermout Sandy, Monod Michel, Losson Bertrand, Mignon Bernard
Department of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liège, Liège, Belgium.
Med Mycol. 2003 Dec;41(6):495-501. doi: 10.1080/13693780310001615385.
In order to better understand the host-fungus relationship in Microsporum canis dermatophytosis and to identify major fungal antigens, the immune response to a crude exoantigen preparation and to a purified recombinant keratinolytic metalloprotease (r-MEP3) was evaluated in guinea pigs experimentally infected with M. canis. Humoral and cellular immune responses were assessed from day 0 to day 57 post-infection (PI), the former by enzyme-linked immunosorbent assay (ELISA) and the latter via a lymphocyte proliferation assay. Infected guinea pigs developed humoral and cellular responses to both M. canis exoantigen and r-MEP3, while no specific immune response to these antigens was observed in control animals. This is the first report on the development of both humoral and cell-mediated immune responses to a purified keratinase in M. canis dermatophytosis.
为了更好地理解犬小孢子菌皮肤癣菌病中的宿主 - 真菌关系并鉴定主要真菌抗原，在实验性感染犬小孢子菌的豚鼠中评估了对粗制外抗原制剂和纯化重组角蛋白分解金属蛋白酶（r-MEP3）的免疫反应。在感染后第0天至第57天评估体液和细胞免疫反应，前者通过酶联免疫吸附测定（ELISA）进行，后者通过淋巴细胞增殖测定进行。感染的豚鼠对犬小孢子菌外抗原和r-MEP3均产生体液和细胞反应，而在对照动物中未观察到对这些抗原的特异性免疫反应。这是关于犬小孢子菌皮肤癣菌病中对纯化角蛋白酶产生体液和细胞介导免疫反应的首次报道。
