Zaval'skiĭ L Iu, Marchenko A I, Borovik R V
State Research Center for Applied Microbiology, Ministry of Public Health, Russian Federation, Moscow Oblast, Obolensk, 142279 Russia.
Mikrobiologiia. 2003 May-Jun;72(3):407-13.
The chemotaxis of two pseudomonads, P. putida AZ (Naph+) and P. putida AZ (Naph-), differing in the ability to metabolize naphthalene was studied by the known capillary method of Adler and the densitometric method devised in our laboratory. The migration of P. putida AZ (Naph+) cells toward increasing levels of naphthalene was accompanied by the formation of a migrating front of converted naphthalene. P. putida AZ (Naph-) cells, too, exhibited positive chemotaxis to naphthalene, but they did not form the front of converted naphthalene. The analysis of experimental data in terms of a kinetic model of bacterial chemotaxis showed that the densitometric method is a potential tool for studying bacterial chemotaxis to hydrophobic organic substances.
采用阿德勒的经典毛细管法以及我们实验室设计的光密度法，研究了两种在萘代谢能力上存在差异的假单胞菌，即恶臭假单胞菌AZ（萘+）和恶臭假单胞菌AZ（萘-）的趋化性。恶臭假单胞菌AZ（萘+）细胞向萘浓度增加的方向迁移时，伴随着转化萘迁移前沿的形成。恶臭假单胞菌AZ（萘-）细胞对萘也表现出正向趋化性，但它们没有形成转化萘的前沿。根据细菌趋化性动力学模型对实验数据进行分析表明，光密度法是研究细菌对疏水性有机物质趋化性的一种潜在工具。
