抗 HEL 特异性抗独特型抗体的制备及其功能模拟分析。
Preparation of anti-idiotypic antibodies specific for anti-HEL and analysis of their functional mimicry.
作者信息
Mingyuan L, Yu X, Liying X, Hong L, Zhonghua J, Jiawan M, Daoruo W
机构信息
Department of Microbiology, West China University of Medical Sciences, Chengdu 610041.
出版信息
Chin Med Sci J. 2000 Jun;15(2):124-6.
OBJECTIVE
This study is to investigate the functional mimicry by using anti-idiotypic antibodies of enzymes.
METHODS
Monoclonal anti-idiotypic antibodies against anti-HEL (hen egg-white lysozyme, HEL) antibodies were obtained by fusion of Sp2/0 myeloma cells with spleen cells of syngeneic mice immunized with monoclonal anti-HEL antibodies against HEL's different antigenic epitopes. Then bacteriolysis of the anti-idiotypic antibodies were observed.
RESULTS
Eight hybridomas strains secreting anti-idiotypic antibodies were selected and characterized. It was shown that two of eight anti-idiotypic antibodies secreted by two hybridomas (1A10C9 and 2A11C1B3) could mimic HEL catalytic activity to lyse Micrococcus lysodeikticus and that the catalytic effect of mixed anti-idiotypic antibodies of 1A10C9 and 2A11C1B3 was stronger than that of one of them, but less than HEL.
CONCLUSION
The results demonstrated that the anti-idiotypic antibodies that could mimic enzyme activity existed in the idiotype network during anti-enzymatic immune response.
目的
本研究旨在利用酶的抗独特型抗体研究功能模拟。
方法
通过将Sp2/0骨髓瘤细胞与用针对HEL不同抗原表位的单克隆抗HEL抗体免疫的同基因小鼠的脾细胞融合，获得针对抗HEL（鸡卵清溶菌酶，HEL）抗体的单克隆抗独特型抗体。然后观察抗独特型抗体的溶菌作用。
结果
筛选并鉴定出8株分泌抗独特型抗体的杂交瘤细胞株。结果显示，由两株杂交瘤（1A10C9和2A11C1B3）分泌的8种抗独特型抗体中的两种能够模拟HEL的催化活性裂解溶壁微球菌，且1A10C9和2A11C1B3的混合抗独特型抗体的催化效果强于其中之一，但弱于HEL。
结论
结果表明，在抗酶免疫应答过程中，独特型网络中存在能够模拟酶活性的抗独特型抗体。
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