产超广谱β-内酰胺酶临床分离株中可转移多重抗生素耐药性的分子机制研究
[Study on the molecular mechanism of transferable multiple-antibiotic resistance in extended-spectrum beta-lactamase-producing clinical isolates].
作者信息
Lu Jian, Tang Ying-chun, Wu Ben-quan, Zhang Kou-xing, Zhang Tian-tuo, Bi Xiao-gang, Zhu Jia-xin, Tan Shu-qing
机构信息
Department of Respiratory Medicine, The Third Affiliated Hospital of Sun Yat-sen university, Guangzhou 510630, China.
出版信息
Zhonghua Jie He He Hu Xi Za Zhi. 2003 Apr;26(4):199-202.
OBJECTIVE
To investigate the molecular mechanism of transferable multiple-antibiotic resistance in extended-spectrum beta-lactamases (ESBLs) producing isolates.
METHODS
Antibiotics susceptibility was tested by E-test method, and multi-resistance plasmids were screened and isolated by extracting transformant plasmids. Inserted gene Cassettes of class 1 integron were amplified and analyzed by polymerase chain reaction (PCR) and DNA sequencing.
RESULTS
Eight of the nine ESBL-producing plasmids were found to comprise class 1 integron sequence, of them 7 harbored 1 or 2 antibiotic resistant gene cassettes which encoding resistance to aminoglycosides (aacA4, aadA2 or aadA5), trimethoprim (dhfrA12 or dfrA17), rifampicin (arr-3) and chloramphenicol (cmlA6). The function of these gene cassettes corresponded to the resistance profiles of their electro-transformants.
CONCLUSION
Multi-resistance gene cassettes located on plasmids and mediated by class 1 integron may play an important role in causing the development and dissemination of multiple-antibiotic resistance in ESBL-producing clinical isolates.
目的
研究产超广谱β-内酰胺酶（ESBLs）菌株中可转移多重耐药性的分子机制。
方法
采用E-test法检测抗生素敏感性，通过提取转化体质粒筛选并分离多重耐药质粒。采用聚合酶链反应（PCR）和DNA测序对1类整合子的插入基因盒进行扩增和分析。
结果
9个产ESBLs质粒中有8个含有1类整合子序列，其中7个含有1个或2个抗生素抗性基因盒，这些基因盒编码对氨基糖苷类（aacA4、aadA2或aadA5）、甲氧苄啶（dhfrA12或dfrA17）、利福平（arr-3）和氯霉素（cmlA6）的抗性。这些基因盒的功能与其电转化子的耐药谱相对应。
结论
位于质粒上并由1类整合子介导的多重耐药基因盒可能在产ESBLs临床分离株多重耐药性的产生和传播中起重要作用。
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