[结核分枝杆菌H(37)R(v)感染巨噬细胞后一氧化氮的产生及细胞因子的表达]
[Nitric oxide production and expression of cytokines by macrophages infected by M. tuberculosis H(37)R(v)].
作者信息
Li Chuan-you, Xing Ai-ying, Li Liang, Wei Pan-jian, Gu Shu-xiang, Chen Xiao-you, Duan Hong-fei, Chen Xi, Xie Li, Ma Yu
机构信息
Beijing Tuberculosis & Thoracic Tumor Research Institute, Beijing 101149, China.
出版信息
Zhonghua Jie He He Hu Xi Za Zhi. 2003 Apr;26(4):214-7.
OBJECTIVE
To study nitric oxide (NO) production and cytokine expression by macrophages infected by M. tuberculosis H(37)R(v), and to compare the difference between dead and live M. tuberculosis in the induction of immune responses, and thus to show if dead bacteria could be a possible candidate for new vaccines.
METHODS
Reverse transcription-polymerase chain reaction (RT-PCR) and ELISA were used to measure the production of NO and cytokines in macrophages infected by H(37)R(v).
RESULTS
Macrophages infected by viable M. tuberculosis produced more NO, IL-1, IL-12, IL-18, TNF-alpha and inducible nitric oxide synthases (iNOS), as compared with macrophages infected by dead bacteria. The number of bacteria was also an important factor determining the production of NO and cytokines.
CONCLUSIONS
Viable M. tuberculosis H(37)R(v) can induce the activation of macrophages and the production of more NO and cytokines which play important roles in the host immune response. Heat-killed M. tuberculosis H(37)R(v) failed to induce activation of macrophages and the production of NO and cytokines, which makes it unlikely to be a candidate for vaccine development.
目的
研究结核分枝杆菌H(37)R(v)感染巨噬细胞后一氧化氮（NO）的产生及细胞因子的表达，比较死菌和活菌在诱导免疫反应方面的差异，从而探讨死菌是否可能成为新型疫苗的候选物。
方法
采用逆转录-聚合酶链反应（RT-PCR）和酶联免疫吸附测定（ELISA）检测H(37)R(v)感染巨噬细胞后NO和细胞因子的产生。
结果
与死菌感染的巨噬细胞相比，活菌感染的巨噬细胞产生更多的NO、白细胞介素-1（IL-1）、白细胞介素-12（IL-12）、白细胞介素-18（IL-18）、肿瘤坏死因子-α（TNF-α）和诱导型一氧化氮合酶（iNOS）。细菌数量也是决定NO和细胞因子产生的重要因素。
结论
活菌结核分枝杆菌H(37)R(v)可诱导巨噬细胞活化，产生更多在宿主免疫反应中起重要作用的NO和细胞因子。热灭活的结核分枝杆菌H(37)R(v)不能诱导巨噬细胞活化及NO和细胞因子的产生，因此不太可能成为疫苗研发的候选物。
Zotero 插件