Benedict Mark Q, Robinson Alan S
Entomology Unit, Food and Agriculture Organization/International Atomic Energy Agency Agriculture and Biotechnology Laboratory, Agency's Laboratories Seibersdorf, A-1400 Vienna, Austria.
Trends Parasitol. 2003 Aug;19(8):349-55. doi: 10.1016/s1471-4922(03)00144-2.
Potential applications for reducing transmission of mosquito-borne diseases by releasing genetically modified mosquitoes have been proposed, and mosquitoes are being created with such an application in mind in several laboratories. The use of the sterile insect technique (SIT) provides a safe programme in which production, release and mating competitiveness questions related to mass-reared genetically modified mosquitoes could be answered. It also provides a reversible effect that would be difficult to accomplish with gene introgression approaches. Could new technologies, including recombinant DNA techniques, have improved the success of previous mosquito releases? Criteria for an acceptable transgenic sterile mosquito are described, and the characteristics of radiation-induced sterility are compared with that of current transgenic approaches. We argue that SIT using transgenic material would provide an essentially safe and efficacious foundation for other possible approaches that are more ambitious.
通过释放转基因蚊子来减少蚊媒疾病传播的潜在应用已被提出，并且几个实验室正在按照这一应用目的培育蚊子。无菌昆虫技术（SIT）的应用提供了一个安全方案，在该方案中，与大规模饲养的转基因蚊子相关的生产、释放和交配竞争力问题都能得到解答。它还提供了一种可逆效应，这是基因渗入方法难以实现的。包括重组DNA技术在内的新技术能否提高以往蚊子释放的成功率？本文描述了可接受的转基因不育蚊子的标准，并将辐射诱导不育的特征与当前转基因方法的特征进行了比较。我们认为，使用转基因材料的SIT将为其他更具雄心的可能方法提供一个基本安全且有效的基础。
