用于丙酮丁醇梭菌的灵敏基因表达报告系统和诱导型启动子-阻遏系统的开发。
Development of a sensitive gene expression reporter system and an inducible promoter-repressor system for Clostridium acetobutylicum.
作者信息
Girbal Laurence, Mortier-Barrière Isabelle, Raynaud Frédéric, Rouanet Céline, Croux Christian, Soucaille Philippe
机构信息
Centre de Bioingénierie Gilbert Durand, Laboratoire de Biotechnologie-Bioprocédés, UMR CNRS 5504, UMR INRA 792, INSA, France.
出版信息
Appl Environ Microbiol. 2003 Aug;69(8):4985-8. doi: 10.1128/AEM.69.8.4985-4988.2003.
Abstract
A sensitive gene expression reporter system was developed for Clostridium acetobutylicum ATCC 824 by using a customized gusA expression cassette. In discontinuous cultures, time course profiles of beta-glucuronidase specific activity reflected adequately in vivo dynamic up- and down-regulation of acidogenesis- and/or solventogenesis-associated promoter expression in C. acetobutylicum. Furthermore, a new inducible gene expression system was developed in C. acetobutylicum, based on the Staphylococcus xylosus xylose operon promoter-repressor regulatory system.
摘要
通过使用定制的gusA表达盒，为丙酮丁醇梭菌ATCC 824开发了一种灵敏的基因表达报告系统。在分批培养中，β-葡萄糖醛酸酶比活性的时间进程曲线充分反映了丙酮丁醇梭菌中与产酸和/或溶剂生成相关的启动子表达在体内的动态上调和下调。此外，基于木糖葡萄球菌木糖操纵子启动子-阻遏物调控系统，在丙酮丁醇梭菌中开发了一种新的诱导型基因表达系统。
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