Goode D, Allen V M, Barrow P A
Oxford Brookes University, Headington, Oxford OX3 0BP, United Kingdom.
Appl Environ Microbiol. 2003 Aug;69(8):5032-6. doi: 10.1128/AEM.69.8.5032-5036.2003.
Lytic bacteriophages, applied to chicken skin that had been experimentally contaminated with Salmonella enterica serovar Enteritidis or Campylobacter jejuni at a multiplicity of infection (MOI) of 1, increased in titer and reduced the pathogen numbers by less than 1 log(10) unit. Phages applied at a MOI of 100 to 1,000 rapidly reduced the recoverable bacterial numbers by up to 2 log(10) units over 48 h. When the level of Salmonella contamination was low (< log(10) 2 per unit area of skin) and the MOI was 10(5), no organisms were recovered. By increasing the number of phage particles applied (i.e., MOI of 10(7)), it was also possible to eliminate other Salmonella strains that showed high levels of resistance because of restriction but to which the phages were able to attach.
将裂解性噬菌体以感染复数（MOI）为1应用于经实验感染肠炎沙门氏菌血清型肠炎或空肠弯曲菌的鸡皮上，噬菌体滴度增加，且病原体数量减少不到1个对数（10）单位。以MOI为100至1000应用的噬菌体在48小时内可迅速将可回收的细菌数量减少多达2个对数（10）单位。当沙门氏菌污染水平较低（每单位皮肤面积<对数（10）2）且MOI为10（5）时，未回收任何生物体。通过增加应用的噬菌体颗粒数量（即MOI为10（7）），也有可能消除因限制作用而表现出高抗性但噬菌体能够附着的其他沙门氏菌菌株。
