阳离子聚合物介导的DNA链交换机制研究。

Investigation of DNA strand exchange mechanisms mediated by cationic polymers.

作者信息

Sato Yuichi, Kim Won Jong, Choi Sung Won, Saito Minako, Kano Arihiro, Akaike Toshihiro, Maruyama Atsushi

机构信息

Precursory Research for Embryonic Science and Technology, Japan Science and Technology Corporation, 4-1-8 Honcho, Kawaguti, Saitama 332-0012, Japan.

出版信息

Nucleic Acids Res Suppl. 2002(2):59-60. doi: 10.1093/nass/2.1.59.

DOI:10.1093/nass/2.1.59

PMID:12903104

Abstract

It was observed that conjugation of dextran for PLL provided PLL-g-Dex with new properties of enhancing and accelerating inter-nucleotide recognition (1, 2, 3). In this paper, we report the effects of cationic PLL-g-Dex copolymers on base-pair formation of self-complementary octamer oligonucleotide by using NMR and CD analysis. NMR studies revealed that PLL-g-Dex copolymer did not affect hydrogen-bondings between bases in DNA duplex. CD studies also supported inert nature of the copolymer upon hybrid structure.

摘要

据观察，聚赖氨酸（PLL）与葡聚糖的结合赋予了聚赖氨酸接枝葡聚糖（PLL-g-Dex）增强和加速核苷酸间识别的新特性（1, 2, 3）。在本文中，我们通过核磁共振（NMR）和圆二色性（CD）分析报告了阳离子型聚赖氨酸接枝葡聚糖共聚物对自互补八聚体寡核苷酸碱基对形成的影响。NMR研究表明，聚赖氨酸接枝葡聚糖共聚物不会影响DNA双链体中碱基之间的氢键。CD研究也支持了该共聚物在杂交结构上的惰性。