Department of Life Science and Technology, Tokyo Institute of Technology, Nagatsuta-cho 4259 B-57, Midori, Yokohama 226-8501, Japan.
ACS Appl Mater Interfaces. 2022 Aug 31;14(34):39396-39403. doi: 10.1021/acsami.2c11548. Epub 2022 Aug 16.
Various DNA assembly techniques and structures have emerged with the continuous progress of DNA nanotechnology. DNA hybridization chain reaction (HCR) is a representative example owing to isothermal and enzyme-free features. However, HCR is time consuming and is inhibited by nucleases present in biological samples. Herein, we demonstrated that a cationic copolymer, poly(l-lysine)--dextran (PLL--Dex), significantly facilitated HCR and increased its initiator sensitivity by 40-fold. PLL--Dex promoted the generation of HCR products with high molecular weight by accelerating the initiation and the subsequent growth steps of HCR. Moreover, PLL--Dex protected the HCR system from nucleases, permitting HCR in the presence of serum components. Addition of PLL--Dex is a universal and efficient strategy that does not require optimization of the reactor setup or DNA sequences, thus laying a solid foundation for the wider application of HCR.
随着 DNA 纳米技术的不断发展，出现了各种 DNA 组装技术和结构。DNA 杂交链式反应（HCR）是一个具有代表性的例子，因为它具有等温、无酶的特点。然而，HCR 耗时较长，并且会受到生物样品中存在的核酸酶的抑制。本文中，我们证明了一种阳离子共聚物聚赖氨酸-葡聚糖（PLL-Dex）通过将引发剂的灵敏度提高 40 倍，显著促进了 HCR，并提高了其起始效率。PLL-Dex 通过加速 HCR 的起始和后续的增长步骤，促进了高分子量 HCR 产物的生成。此外，PLL-Dex 还保护 HCR 系统免受核酸酶的影响，允许在含有血清成分的情况下进行 HCR。添加 PLL-Dex 是一种通用且高效的策略，不需要对反应装置或 DNA 序列进行优化，为 HCR 的更广泛应用奠定了坚实的基础。
