通过氨酰-tRNA合成酶网络中tRNA的动力学识别进行翻译质量控制。
Quality control of translation through the kinetic discrimination of tRNAs in the network of aminoacyl-tRNA synthetases.
作者信息
Shimada Nobukazu, Matsuzaki Keiko, Suzuki Takeo, Suzuki Tsutomu, Watanabe Kimitsuna
机构信息
Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
出版信息
Nucleic Acids Res Suppl. 2002(2):79-80. doi: 10.1093/nass/2.1.79.
It is known that each aminoacyl-tRNA synthetase (aaRS) specifically recognizes its cognate tRNAs to ensure the correct translation of the genetic information. However, we had previously demonstrated that mammalian mitochondrial seryl-tRNA synthetase (mt SerRS) can markedly misacylate mitochondrial (mt) tRNA(Gln). To investigate extensively misacylation reactions in mammalian mitochondrion, we purified overall twenty-two mt tRNAs from bovine liver, and determined their misacylation activities using mt SerRS. In addition to tRNA(Gln), tRNA(Ala) and tRNA(Asn) showed weak but significant serylation activities, which raises the possibility that each mammalian mt aaRS can misacylate several non-cognate mt tRNAs in varying degrees, but translational fidelity might be maintained by kinetic discrimination of tRNAs in the network of aaRSs.
已知每种氨酰 - tRNA合成酶（aaRS）都能特异性识别其对应的tRNA，以确保遗传信息的正确翻译。然而，我们之前已经证明，哺乳动物线粒体丝氨酰 - tRNA合成酶（mt SerRS）能够显著地将线粒体（mt）tRNA（Gln）错误氨酰化。为了广泛研究哺乳动物线粒体中的错误氨酰化反应，我们从牛肝中纯化了总共22种mt tRNA，并使用mt SerRS测定了它们的错误氨酰化活性。除了tRNA（Gln）外，tRNA（Ala）和tRNA（Asn）也表现出较弱但显著的丝氨酰化活性，这增加了一种可能性，即每种哺乳动物mt aaRS都可能在不同程度上使几种非对应mt tRNA发生错误氨酰化，但翻译保真度可能通过aaRS网络中tRNA的动力学识别得以维持。
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