根癌农杆菌MAFF301001基因组中四个核糖体RNA操纵子的特征分析。

Characterization of four ribosomal RNA operons in the genome of Agrobacterium tumefaciens MAFF301001.

作者信息

Bautista-Zapanta J-ney, Suzuki Katsunori, Yoshida Kazuo

机构信息

Laboratory of Plant Molecular Biology and Bioinformatics, Department of Biological Science, Graduate School of Science, Hiroshima University, Kagamiyama 1-3-1, Higashi Hiroshima Shi, 739-8526 Japan.

出版信息

Nucleic Acids Res Suppl. 2002(2):91-2. doi: 10.1093/nass/2.1.91.

DOI:10.1093/nass/2.1.91

PMID:12903120

Abstract

The four ribosomal RNA (rrn) operons rrnA, rrnB, rrnC and were identified, sequenced completely and characterized individually rrnD in the whole genome of A tumefaciens MAFF301001. These rrn operons were located in the two topologically different chromosomal DNAs; rrnA and rrnD in the linear chromosome and rrnB and rrnC in the circular chromosome. Each operon coded for three ribosomal RNA subunit genes; 16S, 23S and 5S rRNA. The 16S-23S internal transcribed spacers (ITS) of the four rrn operons contain genes for tRNA-Ile and tRNA-Ala and tRNA-Met downstream of 5S rDNA gene while the intergenic spacer between 23S rDNA and 5S rDNA lacked tRNA genes. Sequence alignment of 23S rRNAs of A. tumefaciens MAFF301001 and C58 strains showed unrelated sequences near the 5' end region suggesting the presence of intervening sequence (IVS). Primer extension analyses revealed that the primary transcription products for 16S and 23S rRNAs are 1497 and 2877 bases long, respectively.

摘要

在根癌土壤杆菌MAFF301001的全基因组中鉴定出了四个核糖体RNA（rrn）操纵子rrnA、rrnB、rrnC和rrnD，对它们进行了完整测序并分别进行了特征分析。这些rrn操纵子位于两种拓扑结构不同的染色体DNA中；rrnA和rrnD在线性染色体上，rrnB和rrnC在环状染色体上。每个操纵子编码三个核糖体RNA亚基基因；16S、23S和5S rRNA。四个rrn操纵子的16S - 23S内部转录间隔区（ITS）在5S rDNA基因下游包含tRNA - Ile、tRNA - Ala和tRNA - Met的基因，而23S rDNA和5S rDNA之间的基因间隔区缺乏tRNA基因。根癌土壤杆菌MAFF301001和C58菌株的23S rRNA序列比对显示，在5'端区域附近序列不相关，表明存在间隔序列（IVS）。引物延伸分析表明，16S和23S rRNA的初级转录产物分别长1497和2877个碱基。