Dryden S C, Kaplan S
Department of Microbiology, University of Texas Health Science Center, Houston 77225.
Nucleic Acids Res. 1990 Dec 25;18(24):7267-77. doi: 10.1093/nar/18.24.7267.
We have identified, cloned and sequenced the three ribosomal RNA (rRNA) operons (rrn) present in the facultative photoheterotroph Rhodobacter sphaeroides. DNA sequence analysis has identified the 16S, 23S, and 5S rRNAs, two tRNAs (ile and ala) in the spacer region between the 16S and 23S rRNAs, and an f-met tRNA immediately following the 5S rRNA gene of all three operons. Physical mapping, genetic analysis, and Southern hybridization data indicate that rrnA is contained on a large chromosome and rrnB and rrnC are contained on a second smaller chromosome. These findings are discussed in relation to the origins of diploidy.
我们已经鉴定、克隆并测序了兼性光合异养菌球形红细菌中存在的三个核糖体RNA（rRNA）操纵子（rrn）。DNA序列分析确定了16S、23S和5S rRNA，在16S和23S rRNA之间的间隔区有两个tRNA（ile和ala），并且在所有三个操纵子的5S rRNA基因之后紧接着一个f-met tRNA。物理图谱、遗传分析和Southern杂交数据表明，rrnA位于一条大染色体上，rrnB和rrnC位于第二条较小的染色体上。结合二倍体的起源对这些发现进行了讨论。
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