Ali Md Monsur, Nagatsugi Fumi, Yamamoto Fumihiko, Maeda Minoru, Sasaki Shigeki
Graduate School of Pharmaceutical Sciences, Kyushu University and CREST (JST), 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
Nucleic Acids Res Suppl. 2002(2):149-50. doi: 10.1093/nass/2.1.149.
We have previously demonstrated that 2-amino-6-vinylpurine is an efficient cross-linking agent with high selectivity towards cytidine. In this study, we applied this nucleobase analog to versatile conjugation of oligodeoxynucleotides with radio-, spin-, fluorescence- and peptide labels. The oligodeoxynucleotides incorporating 2-amino-6-vinylpurine nucleoside were reacted with amino- bearing nucleophies in an excess amount to give the corresponding conjugates in good yield.
我们之前已经证明，2-氨基-6-乙烯基嘌呤是一种对胞嘧啶具有高选择性的高效交联剂。在本研究中，我们将这种核碱基类似物应用于寡脱氧核苷酸与放射性、自旋、荧光和肽标签的多功能偶联。将含有2-氨基-6-乙烯基嘌呤核苷的寡脱氧核苷酸与过量的含氨基亲核试剂反应，以良好的产率得到相应的偶联物。
