Taki Masumi, Hohsaka Takahiro, Murakami Hiroshi, Kuno Atsushi, Hasegawa Tsunemi, Taira Kazunari, Sisido Masahiko
Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University, 3-1-1 Tsushimanaka, Okayama 700-8530, Japan.
Nucleic Acids Res Suppl. 2002(2):203-4. doi: 10.1093/nass/2.1.203.
A novel highly fluorescent nonnatural amino acid (beta-(N-methylanthraniloyl)-L-alpha,beta-diaminopropionic acid; nmaDap) was incorporated at the 120th position of streptavidin. The position was directed by a CGGG/CCCG four-base codon/anticodon pair. The incorporation efficiency and the biotin-binding ability of the nmaDap mutant were compared to those of beta-anthraniloyl-L-alpha,beta-diaminopropionic acid (atnDap).
一种新型的高荧光非天然氨基酸（β-（N-甲基邻氨基苯甲酰基）-L-α，β-二氨基丙酸；nmaDap）被掺入到链霉亲和素的第120位。该位置由CGGG/CCCG四碱基密码子/反密码子对引导。将nmaDap突变体的掺入效率和生物素结合能力与β-邻氨基苯甲酰基-L-α，β-二氨基丙酸（atnDap）的进行了比较。
