Mitsui Tsuneo, Kimoto Michiko, Harada Yoko, Sato Akira, Kitamura Aya, To Taiko, Hirao Ichiro, Yokoyama Shigeyuki
Research Center for Advanced Science and Technology, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.
Nucleic Acids Res Suppl. 2002(2):219-20. doi: 10.1093/nass/2.1.219.
A hydrophobic unnatural nucleoside of 4-propynylpyrrole-2-carbaldehyde (designated as Pa') was synthesized to improve its affinity with a pairing partner, 9-methyl-imidazo[(4,5)-b]pyridine (Q), in enzymatic incorporation. In single-nucleotide insertion experiments using the Klenow fragment, the substrate of Pa' (dPa'TP) was efficiently incorporated opposite Q in the template strand, as compared to the incorporation of pyrrole-2-carbaldehyde (dPaTP), which was previously developed.
合成了一种4-丙炔基吡咯-2-甲醛的疏水非天然核苷（命名为Pa'），以提高其在酶促掺入中与配对伙伴9-甲基咪唑并[(4,5)-b]吡啶（Q）的亲和力。在使用Klenow片段的单核苷酸插入实验中，与先前开发的吡咯-2-甲醛（dPaTP）的掺入相比，Pa'的底物（dPa'TP）能有效地掺入模板链中与Q相对的位置。
