耐甲氟喹恶性疟原虫的基因组分析。

Genomic analysis of mefloquine-resistant Plasmodium falciparum.

作者信息

Begum Khurshida, Kim Hye-Sook, Okuda Yohei, Wataya Yusuke, Kimura Mikio, Huruta Takahisa

机构信息

Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Okayama 700-8530, Japan.

出版信息

Nucleic Acids Res Suppl. 2002(2):223-4. doi: 10.1093/nass/2.1.223.

DOI:10.1093/nass/2.1.223

PMID:12903186

Abstract

We studied sequence polymorphism, expression level of pfmdr1 gene and drug sensitivity in mefloquine sensitive-strain as well as mefloquine-resistant clone/24 to understand underlying drug resistance mechanism. Mefloquine-resistant clone/24 exhibited decreased susceptibility to mefloquine, quinine, halofantrine and artemisinin and increased susceptibility to chloroquine. We analyzed sequence of pfmdr1 gene and found mutation in pfmdr1 on clone/24. Moreover, overexpression of mRNA level of pfmdr1 has been observed in mefloquine-resistant clone/24. Our results explain gene mutation and expression level of pfmdr1 may be associated in mefloquine resistance mechanism.

摘要

我们研究了甲氟喹敏感株以及甲氟喹耐药克隆/24中的序列多态性、pfmdr1基因的表达水平和药物敏感性，以了解潜在的耐药机制。甲氟喹耐药克隆/24对甲氟喹、奎宁、卤泛群和青蒿素的敏感性降低，而对氯喹的敏感性增加。我们分析了pfmdr1基因的序列，发现在克隆/24的pfmdr1上存在突变。此外，在甲氟喹耐药克隆/24中观察到pfmdr1的mRNA水平过表达。我们的结果表明，pfmdr1的基因突变和表达水平可能与甲氟喹耐药机制有关。