通过核酸酶S1与肽核酸（PNA）联用检测单核苷酸多态性

Detection of single nucleotide polymorphisms by the combination of nuclease S1 and PNA.

作者信息

Ye Sheng, Liang Xingguo, Yamamoto Yoji, Komiyama Makoto

机构信息

Research Center for Advanced Science and Technology, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.

出版信息

Nucleic Acids Res Suppl. 2002(2):235-6. doi: 10.1093/nass/2.1.235.

DOI:10.1093/nass/2.1.235

PMID:12903192

Abstract

A very simple and practical genotyping method has been developed by combining peptide nucleic acid (PNA) and nuclease S1. The portion in the sample DNA, which is complementary with PNA (either completely or partially), is protected from the enzymatic digestion, providing the DNA fragments of predetermined size. The MALDI-TOF MASS on them concretely substantiates whether the SNP exists in the DNA of patient or not. By employing appropriate reaction conditions and using a dye as probe, the SNP diagnosis is visually accomplished by naked eyes.

摘要

通过将肽核酸（PNA）与核酸酶S1相结合，开发出了一种非常简单且实用的基因分型方法。样品DNA中与PNA互补的部分（完全或部分互补）受到保护，免受酶切消化，从而产生预定大小的DNA片段。对这些片段进行基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MASS）分析，具体证实患者DNA中是否存在单核苷酸多态性（SNP）。通过采用适当的反应条件并使用染料作为探针，可通过肉眼直观地完成SNP诊断。