S1核酸酶与肽核酸（PNA）联合用于双链DNA的位点选择性水解。与使用铈（IV）/乙二胺四乙酸（EDTA）进行位点选择性水解的比较。

Combination of S1 nuclease and PNA for site-selective hydrolysis of double-stranded DNA. Comparison with the site-selective hydrolysis using Ce(IV)/EDTA.

作者信息

Yamamoto Yoji, Komiyama Makoto

机构信息

Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.

出版信息

Nucleic Acids Symp Ser (Oxf). 2004(48):149-50. doi: 10.1093/nass/48.1.149.

DOI:10.1093/nass/48.1.149

PMID:17150522

Abstract

The potential of the combination of SI nuclease and pseudo-complementary PNA (pcPNA) for site-selective scission of double-stranded DNA has been investigated. Through strand invasion of two pcPNAs, single-stranded portions were formed in both strands of substrate DNA. In the initial stage of the enzymatic digestion, two scission fragments were obtained due to the hydrolysis at these two gap-like sites. On prolonged reactions, however, these products (as well as the substrate DNA) were further digested to smaller fragments. Under the conditions employed here, only Ce(IV)/EDTA is available for the preparation of desired fragments from double-stranded DNA.

摘要

已对SI核酸酶与假互补肽核酸（pcPNA）组合用于双链DNA位点选择性断裂的潜力进行了研究。通过两个pcPNA的链侵入，在底物DNA的两条链中均形成了单链部分。在酶促消化的初始阶段，由于在这两个间隙样位点的水解作用，获得了两个断裂片段。然而，在延长反应时间后，这些产物（以及底物DNA）被进一步消化成更小的片段。在此处采用的条件下，只有Ce(IV)/EDTA可用于从双链DNA制备所需片段。