Kitamatsu Mizuki, Shigeyasu Masanori, Okada Tomoyuki, Nakai Takashi, Saitou Mamoru, Sisido Masahiko
Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University, Okayama 700-8530, Japan.
Nucleic Acids Res Suppl. 2002(2):261-2. doi: 10.1093/nass/2.1.261.
We have developed a new series of oxy-peptide nucleic acids with pyrrolidine rings of four different types of structural isomers (cis L, cis D, trans L, trans D configurations; POPNAs). Melting curves of adenine nanomers of POPNAs in the presence of the complementary DNA showed the highest Tm value for cis L-POPNA, but in the case of RNA, the highest Tm value was observed for trans L-POPNA. Thermodynamic parameters (delta H and delta S) of POPNA/DNA and POPNA/RNA hybridizations were evaluated from concentration dependence of Tm values. The POPNA/DNA and POPNA/RNA hybridizations were governed by entropy and enthalpy terms, respectively.
我们开发了一系列新的氧代肽核酸，其吡咯烷环具有四种不同类型的结构异构体（顺式L、顺式D、反式L、反式D构型；POPNA）。在存在互补DNA的情况下，POPNA腺嘌呤纳米体的熔解曲线显示顺式L-POPNA的Tm值最高，但对于RNA，反式L-POPNA的Tm值最高。根据Tm值的浓度依赖性评估了POPNA/DNA和POPNA/RNA杂交的热力学参数（ΔH和ΔS）。POPNA/DNA和POPNA/RNA杂交分别由熵项和焓项主导。
