Department of Medical and Bioengineering, Graduate School of Natural Science and Technology, Okayama University, 3-1-1 Tsushimanaka, Kita-ku, Okayama 700-0082, Japan.
Bioorg Med Chem Lett. 2011 Jan 1;21(1):225-7. doi: 10.1016/j.bmcl.2010.11.034. Epub 2010 Nov 11.
To investigate the antisense effect of a pyrrolidine-based oxy-peptide nucleic acid (POPNA), we carried out the LacZ reporter assay using a 12-mer trans-l-POPNA conjugated with a cell-penetrating peptide (antisense reagent). The antisense effect of the conjugated POPNA (inhibition of LacZ activity) was comparable to that shown by a Nielsen-type peptide nucleic acid. Furthermore, the conjugated POPNA could switch the LacZ activity over a wide range of ambient temperatures.
为了研究基于吡咯烷的氧肽核酸（POPNA）的反义效应，我们使用与穿膜肽（反义试剂）偶联的 12 个碱基的反义转-L-POPNA 进行了 LacZ 报告基因检测。偶联的 POPNA 的反义效应（抑制 LacZ 活性）与尼尔森型肽核酸相似。此外，偶联的 POPNA 可以在较宽的环境温度范围内切换 LacZ 活性。
