Liao M, Fang F
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS, PUMC, Beijing 100005, China.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2000 Aug;22(4):388-91.
Yeast one-hybrid system based on the principle of yeast two-hybrid system, is applied for isolating novel genes encoding proteins that bind to a target, cis-acting regulatory element. The system may also be applied to map the DNA-binding domain of previously known, or newly identified DNA-binding proteins. Yeast one-hybrid system offers maximal sensitivity because detection of the DNA-protein interaction occurs while proteins are in their native configurations. In addition, the gene encoding the DNA-binding proteins of interest is immediately available after a library screening. In this mini-review, we introduce the principle, the experimental procedures, the applications, and the merits of yeast one-hybrid system.
酵母单杂交系统基于酵母双杂交系统的原理，用于分离编码与顺式作用调控元件靶标结合的蛋白质的新基因。该系统也可用于绘制已知或新鉴定的DNA结合蛋白的DNA结合结构域。酵母单杂交系统具有最高的灵敏度，因为DNA-蛋白质相互作用的检测是在蛋白质处于天然构象时进行的。此外，在文库筛选后可立即获得编码感兴趣的DNA结合蛋白的基因。在本综述中，我们介绍了酵母单杂交系统的原理、实验步骤、应用及优点。
