Ceglecka M
Zakładu Toksykologii Instytutu Farmakologii, Toksykologii Pomorskiej Akademii Medycznej.
Ann Acad Med Stetin. 1992;38:79-95.
The aim of the study has been to experimentally estimate the chronic exposure of selected biochemical parameters of serum and microsomal level fraction in animals to a mixture of organic solvents. An attempt was made to alleviate the eventual changes by applying Cernitin preparation. The experiment was performed on male rats, Wistar strain. The rats were exposed to the organic solvents in a toxicological chamber with controlled parameters. Cernitin preparation was added to standard diet, being given to the animals in the form of balls. The biochemical investigations were carried out after a lapse of 3 and 6 month exposition. The range of the accomplished studies included: activity of enzymes (AspAT, AlAT, AP, ChE) bilirubin level and lipids content in blood serum. Lipids content was determined in liver homogenate. The content of protein, cholesterol, phospholipids and free fatty acids, was studied in liver microsomes. It has been shown that protracted exposure to the mixture of organic solvents elicits an increase in the activity of the studied aminotransferases and alkaline++ phosphatase, as well as a decrease in the activity of cholesterase. The changes in activity are accompanied by a rise in the content of lipids. Cernitin preparation used prophylactically normalizes impairments affecting the studied enzymatic and lipid parameters.
本研究的目的是通过实验评估动物血清和微粒体水平部分选定生化参数长期暴露于有机溶剂混合物的情况。尝试通过应用塞尔尼通制剂来缓解可能出现的变化。实验在雄性Wistar品系大鼠身上进行。大鼠在参数可控的毒理学舱中暴露于有机溶剂。塞尔尼通制剂添加到标准饮食中，制成丸子喂给动物。在暴露3个月和6个月后进行生化研究。完成的研究范围包括：酶（天冬氨酸氨基转移酶、丙氨酸氨基转移酶、碱性磷酸酶、胆碱酯酶）活性、血清胆红素水平和血脂含量。在肝脏匀浆中测定血脂含量。研究了肝微粒体中蛋白质、胆固醇、磷脂和游离脂肪酸的含量。结果表明，长期暴露于有机溶剂混合物会导致所研究的氨基转移酶和碱性磷酸酶活性增加，以及胆碱酯酶活性降低。活性变化伴随着脂质含量的升高。预防性使用塞尔尼通制剂可使影响所研究的酶和脂质参数的损伤恢复正常。
