Kaneko Takehito, Whittingham David G, Overstreet James W, Yanagimachi Ryuzo
Institute for Biogenesis Research, Department of Anatomy and Reproductive Biology, John A. Burns School of Medicine, University of Hawaii, Honolulu, Hawaii 96822, USA.
Biol Reprod. 2003 Dec;69(6):1859-62. doi: 10.1095/biolreprod.103.019729. Epub 2003 Aug 6.
Mouse spermatozoa from the caudae epididymides could be freeze-dried without losing their ability to support normal development. Immature spermatozoa from the testes, in contrast, were damaged by freeze-drying. However, immature spermatozoa became resistant to freeze-drying after their treatment with diamide, which oxidizes free -SH groups. Conversely, epididymal spermatozoa were damaged by freeze-drying if first treated with dithiothreitol (DTT), which reduces -SS- bonds. The potential for freeze-drying damage seems likely to relate to the -SS- status of sperm proteins, in particular its protamines.
来自附睾尾部的小鼠精子可以进行冻干处理，而不会丧失其支持正常发育的能力。相比之下，来自睾丸的未成熟精子在冻干过程中会受到损伤。然而，未成熟精子在用二酰胺处理后变得对冻干有抗性，二酰胺可氧化游离的 -SH 基团。相反，如果先用二硫苏糖醇（DTT）处理，附睾精子在冻干时会受到损伤，DTT 可还原 -SS- 键。冻干损伤的可能性似乎与精子蛋白的 -SS- 状态有关，尤其是其鱼精蛋白。
