Guo Chuan-Bin, Cui Nian-Hui, Yu Guang-Yan, Liu Ding-Xin, Meng Shu-Cong, Song Qing
Peking University, Beijing, The People's Republic of China.
J Oral Maxillofac Surg. 2003 Aug;61(8):909-12. doi: 10.1016/s0278-2391(03)00237-4.
Inhibition of cerulenin on the endogenous fatty acid synthetic activities of oral squamous cell carcinoma (OSCC) and normal oral mucosa was assayed.
Squamous cell carcinoma and normal oral mucosa were collected fresh from surgical specimens. The collected tissues were minced in RPMI 1640 and divided into 3 groups: cerulenin treated, dimethylsulfoxide treated, and control. The tissues were incubated in [1(2)-(14)C]acetic acid, sodium salt for the last 2.5 hours of the treatment at 37 degrees C in 5% CO(2). After labeling, total lipids were extracted and counted for (14)C by scintillation counting.
Endogenous fatty acid synthetic activities of oral squamous cell caranoma in the cerulenin-treated group decreased by 19% at 1 hour, 64% at 2 hours, and 87% at 4 hours; remained nearly unchanged in the dimethylsulfoxide-treated group; and increased slightly in the control group. The oral mucosa tissues were only mildly affected by cerulenin in fatty acid synthesis.
Cerulenin significantly inhibits fatty acid synthetic activity in squamous cell carcinoma and only mildly affected the oral mucosa, indicating that the fatty acid synthetic pathway may be exploited as a target for developing anticancer drugs.
检测浅蓝菌素对口腔鳞状细胞癌（OSCC）和正常口腔黏膜内源性脂肪酸合成活性的抑制作用。
从手术标本中新鲜采集鳞状细胞癌组织和正常口腔黏膜组织。将采集的组织在RPMI 1640中切碎，分为3组：浅蓝菌素处理组、二甲基亚砜处理组和对照组。在37℃、5%二氧化碳条件下，于处理的最后2.5小时将组织置于[1(2)-(14)C]乙酸钠盐中孵育。标记后，提取总脂质并通过闪烁计数法对(14)C进行计数。
浅蓝菌素处理组口腔鳞状细胞癌的内源性脂肪酸合成活性在1小时时下降了19%，2小时时下降了64%，4小时时下降了87%；二甲基亚砜处理组几乎保持不变；对照组略有增加。浅蓝菌素对口腔黏膜组织的脂肪酸合成仅有轻微影响。
浅蓝菌素显著抑制鳞状细胞癌中的脂肪酸合成活性，对口腔黏膜仅有轻微影响，表明脂肪酸合成途径可作为开发抗癌药物的靶点。
