Wang Yu-Feng, Wang Xiao-Yi, Gao Qing-Hong, Yang Xiao-Yong, Wang Hong-Ping, Zeng Lei
Key Laboratory of Oral Biomedical Engineering of Ministry of Education, Sichuan University, Chengdu 610041, China.
Hua Xi Kou Qiang Yi Xue Za Zhi. 2006 Dec;24(6):551-4.
To relatively quantify the gene expression of fatty acid synthase in squamous cell carcinoma, adjacent tissue, and some normal oral tissues by real-time quantitative PCR.
The tissues were collected fresh from surgical specimens. The collected tissues were minced. Then the total RNA was extracted. The RNA was reversely transcripted into cDNA with random prime. And then the cDNA was amplified by real-time quantitative PCR to quantify the gene expression of FAS according to an internal control GAPDH. The difference of FAS gene expression was compared between squamous cell carcinoma, adjacent tissue, and some normal oral tissues.
The expression of FAS of squamous cell carcinoma was notably higher than the other two (P < 0.001).
Real-time quantitative PCR provides a method for monitoring the expression of fatty acid synthetic activity in squamous cell carcinoma, adjacent and normal tissues.
通过实时定量聚合酶链反应相对定量脂肪酸合酶在鳞状细胞癌、癌旁组织及部分正常口腔组织中的基因表达。
从手术标本中新鲜采集组织。将采集的组织切碎。然后提取总RNA。用随机引物将RNA逆转录成cDNA。接着通过实时定量聚合酶链反应扩增cDNA，以根据内参甘油醛-3-磷酸脱氢酶定量脂肪酸合酶的基因表达。比较鳞状细胞癌、癌旁组织及部分正常口腔组织中脂肪酸合酶基因表达的差异。
鳞状细胞癌中脂肪酸合酶的表达明显高于其他两者（P < 0.001）。
实时定量聚合酶链反应为监测鳞状细胞癌、癌旁及正常组织中脂肪酸合成活性的表达提供了一种方法。
