Geiger Jeremy, Wessels Deborah, Soll David R
Department of Biological Sciences, The University of Iowa, Iowa City, IA 52242, USA.
Cell Motil Cytoskeleton. 2003 Sep;56(1):27-44. doi: 10.1002/cm.10133.
It has been assumed that the natural chemotactic signal that attracts human polymorphonuclear leukocytes (PMNs) over long distances to sites of infection is in the form of a standing spatial gradient of chemoattractant. We have questioned this assumption on the grounds, first, that standing spatial gradients may not be stable over long distances for long periods of time and, second, that in the one animal cell chemotaxis system in which the natural chemotactic signal has been described in space and time, aggregation of Dicytostelium discoideum, the signal is in the form of an outwardly relayed, nondissipating wave of attractant. Here, it is demonstrated that PMNs alter their behavior in each of the four phases of a wave of PMN chemoattractant, fashioned after the Dictyostelium wave, in a manner similar to Dictyostelium. These results demonstrate that PMNs have all of the machinery to respond to a natural wave of attractant, providing support to the hypothesis that the natural signal that attracts PMNs over large distances to sites of infection in the human body may also be in the form of a wave.
一直以来人们认为，吸引人类多形核白细胞（PMN）远距离到达感染部位的天然趋化信号是以趋化因子的固定空间梯度形式存在的。我们对这一假设提出了质疑，理由如下：其一，固定空间梯度在长时间内远距离可能不稳定；其二，在唯一一个已在时空上描述了天然趋化信号的动物细胞趋化系统——盘基网柄菌的聚集过程中，信号是以趋化因子向外传递且不消散的波的形式存在的。在此，我们证明，PMN在模仿盘基网柄菌波形成的PMN趋化因子波的四个阶段中的每一个阶段都会改变其行为，其方式与盘基网柄菌相似。这些结果表明，PMN具备对天然吸引波作出反应的所有机制，为以下假设提供了支持：在人体中，吸引PMN远距离到达感染部位的天然信号也可能是波的形式。
