[应用计算机图像分析技术研究兔角膜上皮及内皮死后间隔时间与DNA含量变化的关系]
[The relationship between the postmortem interval and the changes of DNA content in rabbit's cornea epithelial and endothelium by the computerized image analysis].
作者信息
Liu Cheng-quan, Xiong Ping
机构信息
Department of Forensic Medicine, Nanhua University, Hengyang 421001, China.
出版信息
Fa Yi Xue Za Zhi. 2003;19(2):76-7.
OBJECTIVE
To study the relationship between the postmortem interval (PMI) and the changes of DNA content in rabbit's cornea epithelial and endothelium.
METHODS
Using the computerized image analysis to measure the DNA content in the cornea epithelial and endothelium of 105 rabbit's at every six hours during 72 hours after death.
RESULTS
The degradation rate of DNA in nuclear has an apparent relationship in 72 hours after death of the rabbits.
CONCLUSION
The determination of the quantity of DNA in cornea nuclear is a new method to estimate the PMI accurately.
目的
研究家兔死后角膜上皮及内皮DNA含量变化与死后间隔时间（PMI）的关系。
方法
运用计算机图像分析技术，于家兔死后72小时内每隔6小时测量105只家兔角膜上皮及内皮的DNA含量。
结果
家兔死后72小时内，细胞核内DNA降解速率存在明显相关性。
结论
测定角膜细胞核内DNA含量是准确估计PMI的一种新方法。
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