Long Ren, Wang Wei-ping, Xiong Ping
Department of Forensic Medicine, Nanhua University, Hengyang 421001, China.
Fa Yi Xue Za Zhi. 2005 Aug;21(3):174-6.
To probe the correlation between the postmortem interval (PMI) and the DNA degradation of costicartilage and dental pulp cells in human being after death, and to seek a new method for estimating PMI.
The image cytometry was used to measure the DNA degradation under different ambient temperatures (30-35 degrees C, 15-20 degrees C) in 0-15 days after death.
The average DNA content of two kinds of tissue was degradated with the prolongation of PMI. But there was a plateau period of 0-4 days for dental pulp cells of human being in 15-20 degrees C. There was a high negative correlativity P<0.01 between the average DNA content and PMI.
PMI could be estimated accurately according to the DNA degradation of costicartilage and dental pulp cells in human being after death.
探讨死后间隔时间（PMI）与人死后肋软骨及牙髓细胞DNA降解的相关性，寻求一种估计PMI的新方法。
采用图像细胞仪检测死后0 - 15天在不同环境温度（30 - 35℃、15 - 20℃）下的DNA降解情况。
两种组织的平均DNA含量随PMI延长而降解。但在15 - 20℃环境下，人牙髓细胞在0 - 4天有一个平台期。平均DNA含量与PMI之间存在高度负相关（P<0.01）。
根据人死后肋软骨及牙髓细胞的DNA降解情况可准确估计PMI。
