Liu Ye-hai, Tang Ping-zhang, Xu Zhen-gang, Qi Yong-fa, Ding Fang, Zhang Li-yong, Wang Hai-tao, Liu Zhi-hua
Department of Head and Neck Surgery, Cancer Hospital, CAMS, PUMC, Beijing 100021, China.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2003 Feb;25(1):47-51.
In order to explore pathogenic mechanism of laryngeal carcinoma, the involved genes were identified in larynx carcinogenesis by comparing the gene expression profile in matched primary normal epithelial cells and primary laryngeal carcinoma cells from the same patients.
A cDNA microarray analysis consisting of 11,431 human genes revealed significant changes in the expression of 35 genes, with 8 genes being up-regulated and 27 being down-regulated. The epithelial membrane protein-1 (EMP-1) is one of the down-regulated genes. EMP-1 expression in various kinds of laryngeal carcinoma was determined by semi-quantitative reverse transcriptase polymerase chain reaction (qRT-PCR).
The EMP-1 mRNA levels in all laryngeal carcinoma cells was significantly lower than that in the matched primary normal epithelial cells (P < 0.05) and were not correlated to the stage and differentiation of laryngeal carcinoma (P > 0.05).
The EMP-1 expression was correlated to larynx carcinogenesis and may be helpful to elucidate the pathogenic mechanism in laryngeal carcinoma.
为探讨喉癌的致病机制，通过比较同一患者配对的原发性正常上皮细胞和原发性喉癌细胞中的基因表达谱，确定喉癌发生过程中涉及的基因。
一项包含11431个人类基因的cDNA微阵列分析显示35个基因的表达有显著变化，其中8个基因上调，27个基因下调。上皮膜蛋白-1（EMP-1）是下调基因之一。通过半定量逆转录聚合酶链反应（qRT-PCR）测定EMP-1在各种喉癌中的表达。
所有喉癌细胞中的EMP-1 mRNA水平均显著低于配对的原发性正常上皮细胞（P < 0.05），且与喉癌的分期和分化无关（P > 0.05）。
EMP-1表达与喉癌发生相关，可能有助于阐明喉癌的致病机制。
