Liu Chang, Wei Xiaojun, Li Feng, Wang Li, Ruan Xinjian, Jia Jia, Zhang Xia
Department of Medical Oncology, The Army General Hospital of PLA, Beijing, China (mainland).
Department of General Surgery, The Army General Hospital of PLA, Beijing, China (mainland).
Med Sci Monit. 2017 Aug 5;23:3795-3800. doi: 10.12659/msm.901161.
BACKGROUND In the present study, we aimed to investigate the prognostic value of epithelial membrane protein 1 (EMP-1) gene in patients diagnosed with laryngeal carcinoma (LC). MATERIAL AND METHODS Patients who were pathologically diagnosed with LC were enrolled in the present study. The expression levels of EMP-1 in tumor tissues and corresponding normal tissues collected from the LC patients were detected by semi-reverse transcriptase polymerase chain reaction (semi-RT-PCR). Chi-square analysis was used to evaluate the relationship between EMP-1 expression level and clinical characteristics. Survival analysis for the study population was analyzed by Kaplan-Meier method with log rank test. Additionally, Cox regression model was applied to evaluate the prognostic value of EMP-1 in LC patients. RESULTS 106 LC patients, including 55 men and 51 women, were enrolled in the present study. Semi-RT-PCR demonstrated that the expression level of EMP-1 was decreased in tumor tissues, compared with adjacent normal tissues (p<0.001). Moreover, the level was significantly associated with lymph node metastasis, histological grade, and clinical stage (p<0.05 for all). In addition, low levels of EMP-1 was significantly correlated with poor survival rate (log rank test, p=0.020). Cox regression analysis indicated that EMP-1 was an independent marker for LC prognosis (HR=2.755, 95% CI=1.123-6.760, p=0.027). CONCLUSIONS The abnormal expression of EMP-1 may be associated with progression of LC and the gene may act as a prognostic marker for LC.
背景 在本研究中，我们旨在探讨上皮膜蛋白1（EMP-1）基因在喉癌（LC）患者中的预后价值。
材料与方法 本研究纳入经病理诊断为LC的患者。采用半逆转录聚合酶链反应（semi-RT-PCR）检测LC患者肿瘤组织及相应正常组织中EMP-1的表达水平。采用卡方分析评估EMP-1表达水平与临床特征之间的关系。采用Kaplan-Meier法和对数秩检验对研究人群进行生存分析。此外，应用Cox回归模型评估EMP-1在LC患者中的预后价值。
结果 本研究共纳入106例LC患者，其中男性55例，女性51例。Semi-RT-PCR结果显示，与癌旁正常组织相比，肿瘤组织中EMP-1的表达水平降低（p<0.001）。此外，该水平与淋巴结转移、组织学分级和临床分期显著相关（均p<0.05）。另外，EMP-1低水平与较差的生存率显著相关（对数秩检验，p=0.020）。Cox回归分析表明，EMP-1是LC预后的独立标志物（HR=2.755，95%CI=1.123-6.760，p=0.027）。
结论 EMP-1的异常表达可能与LC的进展有关，该基因可作为LC的预后标志物。
