[人细胞中4种热休克蛋白基因mRNA定量RT-PCR体系的建立与应用]
[Establishment and application of a RT-PCR system for quantifying mRNA of 4 hsp genes in human cells].
作者信息
Li Hong-fan, Liu Xing-rong, Heng Feng-yan, Wu Ning-hua, Shen Yu-fei
机构信息
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
出版信息
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2002 Jun;24(3):321-4.
OBJECTIVE
To establish a RT-PCR system for detecting mRNA expression level of 4 hsp genes in human cells.
METHODS
RT-PCR system was established with gene cloning, gene recombination, in vitro transcription and RT-PCR techniques; The detection of expression level of 4 hsp genes in SW13 cell was carried out with this system.
RESULTS
In SW13 cell, hsp70 and hsp90 alpha were typical heat shock induced genes, while hsp60 and hsp90 beta were efficiently expressed and further induced by heat-shock to various extent.
CONCLUSIONS
In our hands novel RT-PCR system can be used to detect mRNA expression level of 4 human hsp genes.
目的
建立一种用于检测人细胞中4种热休克蛋白（hsp）基因mRNA表达水平的逆转录聚合酶链反应（RT-PCR）系统。
方法
运用基因克隆、基因重组、体外转录及RT-PCR技术建立RT-PCR系统；用该系统检测SW13细胞中4种hsp基因的表达水平。
结果
在SW13细胞中，hsp70和hsp90α是典型的热休克诱导基因，而hsp60和hsp90β高效表达，并在不同程度上受热休克进一步诱导。
结论
我们建立的新型RT-PCR系统可用于检测4种人hsp基因的mRNA表达水平。
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