[体外培养心肌细胞钙化过程中牛磺酸转运变化的观察]
[An observation of taurine transport alterations in calcification of myocardial cells in vitro].
作者信息
Shi Yan-rong, Wang Shu-heng, Bu Ding-fang, Qi Yong-fen, Gao Lin, Pang Yong-zheng, Tang Chao-shu
机构信息
Institute of Cardiovascular Disease Reserch, First Hospital, Peking University, Beijing 100034, China.
出版信息
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2002 Aug;24(4):359-63.
OBJECTIVE
To observe the alterations of taurine transport, taurine transporter (TAUT) and cysteine sulfinate decarboxylase (CSD) mRNA in the calcification of myocardial cells in vitro.
METHODS
3H-taurine measured the amount of taurine uptake. TAUT and CSD mRNA consents were measured using competitive quantitative RT-PCR in cultured and calcified myocardial cells.
RESULTS
In calcification of myocardial cells, taurine concentration was decreased by 27% (P < 0.05), taurine uptake was markedly reduced, Vmax reduced by 39% (P < 0.01), there were no statistical significance of Km values between the two groups. TAUT mRNA decreased by 45% (P < 0.01), but CSD mRNA increased by 25% (P < 0.05).
CONCLUSIONS
The data suggest that there were impediment of taurine transport in calcification of myocardial cells, as TAUT mRNA level was decreased, but CSD mRNA concentration was improved.
目的
观察体外培养心肌细胞钙化过程中牛磺酸转运、牛磺酸转运体（TAUT）及半胱氨酸亚磺酸脱羧酶（CSD）mRNA的变化。
方法
采用3H-牛磺酸检测牛磺酸摄取量。运用竞争性定量逆转录聚合酶链反应（RT-PCR）检测培养及钙化心肌细胞中TAUT和CSD mRNA含量。
结果
心肌细胞钙化时，牛磺酸浓度降低27%（P<0.05），牛磺酸摄取显著减少，最大摄取速率（Vmax）降低39%（P<0.01），两组间米氏常数（Km）值无统计学意义。TAUT mRNA降低45%（P<0.01），而CSD mRNA升高25%（P<0.05）。
结论
数据表明，心肌细胞钙化过程中存在牛磺酸转运障碍，TAUT mRNA水平降低，但CSD mRNA浓度升高。
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