牛磺酸转运体在血管平滑肌细胞中表达。

Taurine transporter is expressed in vascular smooth muscle cells.

作者信息

Liao X-B, Zhou X-M, Li J-M, Tan Z-P, Liu L-M, Zhang W, Tan H, Lu Y, Yuan L-Q

机构信息

Department of Cardiothoracic Surgery, The Second Xiang-Ya Hospital, Central South University, Changsha, Hunan, China.

出版信息

Amino Acids. 2007 Nov;33(4):639-43. doi: 10.1007/s00726-006-0486-8. Epub 2007 Jan 26.

DOI:10.1007/s00726-006-0486-8

PMID:17252307

Abstract

The regulation of vascular smooth muscle cells (VSMCs) function by taurine has been a subject of increasing interest and investigation, and taurine is taken up into cells through a specific transporter system, the taurine transporter (TAUT). In the present study, we examined the expression of TAUT in VSMCs and the kinetic parameters of the uptake process of TAUT in VSMCs. RT-PCR and western blot demonstrated that the mRNA and protein of TAUT was expressed in VSMCs in vitro. Immunohistochemistry using antibody for TAUT revealed the expression of this protein in rat thoracic aorta. The maximal [(3)H]taurine uptake rate in VSMCs was 37.75 +/- 3.13 pmol/min per mg of protein, with a K ( m ) value of 5.42 +/- 0.81 microM. Thus, VSMCs are able to express a functional taurine transporter. The regulation and detailed function of taurine and TAUT in VSMCs remain unclear, but our findings suggest a functional role for them in VSMCs metabolism.

摘要

牛磺酸对血管平滑肌细胞（VSMCs）功能的调节一直是人们越来越感兴趣并深入研究的课题，牛磺酸通过一种特定的转运系统——牛磺酸转运体（TAUT）进入细胞。在本研究中，我们检测了VSMCs中TAUT的表达以及VSMCs中TAUT摄取过程的动力学参数。逆转录聚合酶链反应（RT-PCR）和蛋白质印迹法表明，TAUT的信使核糖核酸（mRNA）和蛋白质在体外培养的VSMCs中表达。使用TAUT抗体进行免疫组织化学显示，该蛋白在大鼠胸主动脉中表达。VSMCs中最大的[³H]牛磺酸摄取率为每毫克蛋白质37.75±3.13皮摩尔/分钟，米氏常数（Km）值为5.42±0.81微摩尔。因此，VSMCs能够表达一种功能性的牛磺酸转运体。牛磺酸和TAUT在VSMCs中的调节及详细功能仍不清楚，但我们的研究结果表明它们在VSMCs代谢中具有功能性作用。