Zhang Lin, Cui Liang, Miao Guo-bin, Zhao Wen-shu, Wang Shu-yan, Liu Xiu-lan
Capital Medical University, Beijing Chao Yang Hospital, Beijing 100020, China.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2002 Aug;24(4):367-9.
To determine whether autoantibodies against the cardiac G-protein-coupled beta 2- and alpha 1-adrenergic and AT1 receptors are related to patients with primary hypertension.
Synthetic peptides corresponding to amino acid sequences of the second extracellular loops of the beta 2- and alpha 1-adrenergic and AT1 receptors were respectively used as antigens to screen sera from patients with hypertensive heart diseases (n = 50) as well as simple hypertension (n = 40) and healthy blood donors (n = 40) using ELISA test.
The positive ratio of autoantibodies against beta 2 and alpha 1 and AT1 receptors in patients with hypertensive heart diseases were significantly higher than patients with simple hypertension and healthy donors. The geometric mean titers of autoantibodies against beta 2- and alpha 1-adrenergic and AT1 receptors had no difference between the patients with hypertensive heart diseases and the patients with simple hypertension, but the geometric mean titers of two groups were higher than healthy donors. In the patients with hypertensive heart diseases, 81.0% of the patients with autoantibodies against beta 2-adrenergic receptor had autoantibodies against alpha 1-adrenergic receptor and 76.2% had autoantibodies against AT1 receptors. The percent of the autoantibodies against three receptors in patients with hypertensive heart diseases were 52.4%.
Autoantibodies against beta 2- and alpha 1-adrenergic and AT1 receptors play an important role in the pathophysiological changes of primary hypertension, and may participate myocardial and vessel remodeling.
确定抗心脏G蛋白偶联β2-、α1-肾上腺素能受体及血管紧张素Ⅱ1型受体（AT1受体）自身抗体是否与原发性高血压患者相关。
分别以对应β2-、α1-肾上腺素能受体及AT1受体第二个细胞外环氨基酸序列的合成肽为抗原，采用酶联免疫吸附测定法（ELISA）检测高血压性心脏病患者（n = 50）、单纯高血压患者（n = 40）及健康献血者（n = 40）的血清。
高血压性心脏病患者抗β2-、α1-及AT1受体自身抗体的阳性率显著高于单纯高血压患者及健康献血者。高血压性心脏病患者与单纯高血压患者抗β2-、α1-肾上腺素能受体及AT1受体自身抗体的几何平均滴度无差异，但两组的几何平均滴度均高于健康献血者。在高血压性心脏病患者中，81.0%抗β2-肾上腺素能受体自身抗体阳性的患者同时有抗α1-肾上腺素能受体自身抗体，76.2%有抗AT1受体自身抗体。高血压性心脏病患者中抗三种受体自身抗体的比例为52.4%。
抗β2-、α1-肾上腺素能受体及AT1受体自身抗体在原发性高血压的病理生理变化中起重要作用，可能参与心肌及血管重塑。
