Tang Yong, Luan Jie, Wang Qian
South 2 Ward, Plastic Surgery Hospital, PUMC, CAMS, Beijing 100041, China.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2002 Aug;24(4):413-7.
To explore the application of ultraviolet spectrophotometer in determining papaverine hydrochloride content in blood and skin and to determine the intradermal content after local external application of papaverine hydrochloride.
Phosphate buffer solution (pH = 10, ionic strength = 0.4) was added into the blood or ground skin tissue containing papaverine hydrochloride, and papaverine hydrochloride was extracted with chloroform-hexane mixture (ratio:2:3). After oscillation and centrifugation, partial organic phase was retrieved and dried in 60 degrees C water bath. The dried product was dissolved in 1 mol/L HCl and then underwent filtration through 0.2 micron membrane. The ultraviolet apectrophotometry was applied to determine the content of papaverine hydrochloride in the filtrate. 2% papaverine hydrochloride cream was applied on pig's skin and skin specimen was retrieved at given time to determine the papaverine hydrochloride content in skin tissue with ultraviolet spectrophotometry.
The absorbance spectrum of the extract was typical of that of papaverine hydrochloride, with no miscellaneous peak. The extractive rate of papaverine hydrochloride in blood of was more than 92% and that in skin tissue more than 87%. Eight hours after external application of 2% papaverine hydrochloride cream on pig skin, the content of papaverine hydrochloride in skin tissue was more than 90 micrograms/g, significantly higher than the effective concentration of papaverine hydrochloride.
Ultraviolet spectrophotometry is a simple, economical and reliable method for the determination of papaverine hydrochloride content in blood and skin tissue, with good reproducibility and sensibility. External application of papaverine hydrocholine cream can provide efficient permeation and drug concentration in the skin.
探讨紫外分光光度计在测定血液及皮肤中盐酸罂粟碱含量的应用，并测定局部外用盐酸罂粟碱后的皮内含量。
向含盐酸罂粟碱的血液或研磨后的皮肤组织中加入磷酸盐缓冲溶液（pH = 10，离子强度 = 0.4），用氯仿 - 己烷混合液（比例：2:3）提取盐酸罂粟碱。振荡离心后，取部分有机相于60℃水浴干燥。干燥产物用1mol/L盐酸溶解，然后经0.2μm滤膜过滤。采用紫外分光光度法测定滤液中盐酸罂粟碱的含量。将2%盐酸罂粟碱乳膏涂抹于猪皮肤，在规定时间取皮肤标本，用紫外分光光度法测定皮肤组织中盐酸罂粟碱的含量。
提取物的吸收光谱为典型的盐酸罂粟碱吸收光谱，无杂峰。盐酸罂粟碱在血液中的提取率大于92%，在皮肤组织中的提取率大于87%。在猪皮肤外用2%盐酸罂粟碱乳膏8小时后，皮肤组织中盐酸罂粟碱含量大于90μg/g，显著高于盐酸罂粟碱的有效浓度。
紫外分光光度法是一种简单、经济、可靠的测定血液及皮肤组织中盐酸罂粟碱含量的方法，重现性和灵敏度良好。外用盐酸罂粟碱乳膏可使药物在皮肤中有效渗透并达到有效浓度。
