Luan Jie, Tang Yong, Yang Peiying, Zhang Xuhui, Hou Dianju, Ma Guie, Wang Qian
Plastic Surgery Hospital, Chinese Academy of Medical Science, Beijing 100041, China.
Zhonghua Zheng Xing Wai Ke Za Zhi. 2002 Jan;18(1):29-32.
To evaluate the effect of external use of papaverine cream on enhancement of tissue expansion.
8 mini pigs were randomly divided into 4 groups. During inflation, papaverine was given externally in group A, through a delivery system in group B, though intramuscular injection in group C. Papaverine was not given in group D. The time of inflation, the expansion rate, the surviving length of expanded flaps and the papaverine concentration in the fibrous capsule in each group were examined. Histologic and electroscope examinations of the expanded tissue were made.
The average inflation time was 28.9 +/- 4.5 days in group A, 34.0 +/- 2.6 days in group B, 37.6 +/- 4.8 days in group C and 38.5 +/- 3.5 days in group D. There was significant difference between each group(P < 0.05). The average surviving length of expanded flaps in group A was 13.67 +/- 1.28 cm, 11.07 +/- 0.88 cm in group B, 10.79 +/- 0.49 cm in group C, and 9.49 +/- 0.77 cm in group D. There was significant difference between each group (P < 0.01). Histologic examinations of the expanded tissue suggested that there were no significant differences in thickness of epidermis, derma and fibrous capsule among the four groups. Electroscope examinations of the myofibroblast in fibrous capsule showed that there were smaller nuclei, fewer microfilaments and fewer mitochondrias and rougher endoplastic reticulume in group A than those in other groups. The concentration of papaverine in fibrous capsule of group A was 3.5 +/- 1.2 micrograms/g. Papaverine was detected neither in the skin of other groups nor in the blood of all groups.
评估外用罂粟碱乳膏对增强组织扩张的效果。
8只小型猪随机分为4组。在注水扩张过程中，A组外用罂粟碱，B组通过给药系统给药，C组肌肉注射，D组不给药。观察各组注水扩张时间、扩张速率、扩张皮瓣存活长度及纤维囊内罂粟碱浓度，并对扩张组织进行组织学及电镜检查。
A组平均注水扩张时间为28.9±4.5天，B组为34.0±2.6天，C组为37.6±4.8天，D组为38.5±3.5天。各组间差异有统计学意义（P<0.05）。A组扩张皮瓣平均存活长度为13.67±1.28cm，B组为11.07±0.88cm，C组为10.79±0.49cm，D组为9.49±0.77cm。各组间差异有统计学意义（P<0.01）。组织学检查显示，4组表皮、真皮及纤维囊厚度差异无统计学意义。电镜检查纤维囊内肌成纤维细胞，A组细胞核较小，微丝、线粒体较少，内质网较粗糙，与其他组相比有差异。A组纤维囊内罂粟碱浓度为3.5±1.2μg/g。其他组皮肤及所有组血液中均未检测到罂粟碱。
