利用酵母双杂交系统寻找G蛋白途径中的新型蛋白质因子
[Hunting for novel protein factors in G-protein pathway with yeast two-hybrid system].
作者信息
Lu N, Li J J, Huang B R
机构信息
Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, CAMS, PUMC, Beijing 100005, China.
出版信息
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2001 Apr;23(2):111-4.
OBJECTIVE
To explore the protein factors that could interact with G beta subunit within the G protein signal transducing pathway.
METHODS
The highly sensitive protein-protein interaction system--Yeast Two-hybrid System was applied to screen the human cDNA library with constructed "Bite plasmid" containing G beta subunit gene fragment. And then the false positive test was adapted.
RESULTS
Three positive gene fragments were obtained. One codes for "Actin bundling protein". The other two are new ones and their Gene Bank accession numbers are AF288405 and AF288406 respectively.
CONCLUSIONS
It is the first time to find that among human brain tissue, G beta subunit becomes a structural or functional unit interacts with actin bundling protein and the other two unknown protein factors to activate the following pathway. This result may be important to understand the relationship between the actin cytoskeleton and G proteins.
目的
探索在G蛋白信号转导途径中能与Gβ亚基相互作用的蛋白质因子。
方法
应用高灵敏度的蛋白质-蛋白质相互作用系统——酵母双杂交系统，用构建的含Gβ亚基基因片段的“诱饵质粒”筛选人cDNA文库，然后进行假阳性检测。
结果
获得3个阳性基因片段。一个编码“肌动蛋白成束蛋白”。另外两个是新的，它们的基因库登录号分别为AF288405和AF288406。
结论
首次发现在人脑组织中，Gβ亚基成为与肌动蛋白成束蛋白及另外两个未知蛋白质因子相互作用的结构或功能单位，从而激活后续途径。该结果对于理解肌动蛋白细胞骨架与G蛋白之间的关系可能具有重要意义。
Zotero 插件