Zhou J Y, Hu S S, Ren B, Qi G Q
Division of Cardiac Valve, Fuwai Cardiovascular Institute, CAMS, PUMC, Beijing 100037, China.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2001 Apr;23(2):193-5.
To obtain an acellular matrix from bovine pericardial tissue, as a scaffold for tissue engineering of heart valves or cardiovascular patching applications.
A four step detergent and enzyme link extraction procedure was practiced in this study. The mechanic properties of the acellular matrix were evaluated, and the components were analyzed biochemically.
HE staining confirmed the removal of cells and Von Gieson staining showed the integrality of collagen and elastin. Biochemical analysis demonstrated the retention of collagen and some glycosaminoglycans while the percentage of the soluble proteins reduced slightly. The tissue fracture strength and shrinkage temperature made no significant difference after extraction.
The extraction process is effective to remove cells from bovine pericardial tissue while maintains its mechanical strength. This approach may eventually lead to a scaffold for heart valves and cardiovascular patching applications in tissue engineering.
从牛心包组织中获取脱细胞基质，作为心脏瓣膜组织工程或心血管修补应用的支架。
本研究采用四步去污剂和酶联提取程序。评估脱细胞基质的力学性能，并对其成分进行生化分析。
苏木精-伊红染色证实细胞已被去除，冯·吉森染色显示胶原蛋白和弹性蛋白完整。生化分析表明胶原蛋白和一些糖胺聚糖得以保留，而可溶性蛋白的百分比略有降低。提取后组织断裂强度和收缩温度无显著差异。
该提取过程能有效去除牛心包组织中的细胞，同时保持其机械强度。这种方法最终可能会为组织工程中的心脏瓣膜和心血管修补应用带来一种支架。
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