Braile Biomédica Indústria Comércio e Representações SA, São José do Rio Preto, São Paulo, Brazil.
Artif Organs. 2011 May;35(5):484-9. doi: 10.1111/j.1525-1594.2011.01264.x.
Major problems with biological heart valves post-implantation are associated with progressive structural deterioration and calcification attributed to glutaraldehyde processing, dead cells, and cell fragments present in the native tissue. In spite of these problems, glutaraldehyde still is the reagent of choice. The results with acellular matrix xenograft usually prepared by detergent treatment in association with enzymes are rather conflicting because while preserving mechanical properties, tissue morphology and collagen structure are process dependent. This work describes a chemical approach for the preparation of an acellular bovine pericardium matrix intended for the manufacture of heart valve bioprostheses. Cell removal was performed by an alkaline extraction in the presence of calcium salts for periods ranging from 6 to 48 h. The results showed that cell removal was achieved after 12 h, with swelling and negative charge increasing with processing time. Nevertheless, collagen fibril structure, ability to form fibrils, and stability to collagenase were progressive after 24-h processing. There was no denaturation of the collagen matrix. A process is described for the preparation of acellular bovine pericardium matrices with preserved fibril structure and morphology for the manufacture of cardiac valve bioprostheses and may be used in other applications for tissue reconstruction.
生物心脏瓣膜植入后的主要问题与渐进性结构恶化和钙化有关，这些问题归因于戊二醛处理、天然组织中存在的死细胞和细胞碎片。尽管存在这些问题，戊二醛仍然是首选试剂。用去污剂处理联合酶处理制备的去细胞基质异种移植物的结果相当矛盾，因为在保持机械性能的同时，组织形态和胶原结构取决于处理过程。本工作描述了一种用于制备用于制造心脏瓣膜生物假体的去细胞牛心包基质的化学方法。细胞去除是通过在存在钙盐的情况下进行碱性提取来实现的，处理时间从 6 小时到 48 小时不等。结果表明，在 12 小时后即可实现细胞去除，随着处理时间的延长，肿胀和负电荷增加。然而，胶原纤维结构、形成纤维的能力以及对胶原酶的稳定性在 24 小时处理后逐渐增强。胶原基质没有变性。描述了一种用于制备具有保留的纤维结构和形态的去细胞牛心包基质的方法，用于制造心脏瓣膜生物假体，并可用于组织重建的其他应用。
