Courtman D W, Pereira C A, Kashef V, McComb D, Lee J M, Wilson G J
Centre for Biomaterials, University of Toronto, Ontario, Canada.
J Biomed Mater Res. 1994 Jun;28(6):655-66. doi: 10.1002/jbm.820280602.
There is evidence to suggest that the cellular components of homografts and bioprosthetic xenografts may contribute to calcification or immunogenic reactions. A four-step detergent and enzymatic extraction process has been developed to remove cellular components from bovine pericardial tissue. The process results in an acellular matrix material consisting primarily of elastin, insoluble collagen, and tightly bound glycosaminoglycans. Light and electron microscopy confirmed that nearly all cellular constituents are removed without ultrastructural evidence of damage to fibrous components. Collagen denaturation temperatures remained unaltered. Biochemical analysis confirmed the retention of collagen and elastin and some differential extraction of glycosaminoglycans. Low strain rate fracture testing and high strain rate viscoelastic characterization showed that, with the exception of slightly increased stress relaxation, the mechanical properties of the fresh tissue were preserved in the pericardial acellular matrix. Crosslinking of the material in glutaraldehyde or poly(glycidyl ether) produced mechanical changes consistent with the same treatments of fresh tissue. The pericardial acellular matrix is a promising approach to the production of biomaterials for heart valve or cardiovascular patching applications.
有证据表明，同种移植物和生物假体异种移植物的细胞成分可能会导致钙化或免疫反应。已开发出一种四步洗涤剂和酶提取工艺，用于从牛心包组织中去除细胞成分。该工艺产生一种主要由弹性蛋白、不溶性胶原蛋白和紧密结合的糖胺聚糖组成的无细胞基质材料。光学显微镜和电子显微镜证实，几乎所有细胞成分都被去除，且纤维成分没有超微结构损伤的证据。胶原蛋白变性温度保持不变。生化分析证实胶原蛋白和弹性蛋白得以保留，且糖胺聚糖有一些差异提取。低应变率断裂测试和高应变率粘弹性表征表明，除了应力松弛略有增加外，新鲜组织的力学性能在心包无细胞基质中得以保留。材料在戊二醛或聚（缩水甘油醚）中的交联产生了与新鲜组织相同处理一致的力学变化。心包无细胞基质是一种有前途的方法，可用于生产用于心脏瓣膜或心血管修补应用的生物材料。
