Zhang Liguo, Pan Jilun, Li Jieliang, Wu Wei, Yu Yaoting
Key Laboratory of Bioactive Materials Ministry of Education, Institute of Molecular Biology, Nankai University, Tianjin, PR China.
Artif Cells Blood Substit Immobil Biotechnol. 2003 Aug;31(3):293-301. doi: 10.1081/bio-120023159.
Chitosan microcarriers were prepared using oxidized lactose as a cross-linking agent which was oxidized by sodium periodate. The effect of amount of oxidized lactose on the preparation of chitosan microcarriers was studied and optimized. Rat hepatocytes cultivated on chitosan microcarriers cross-linked by oxidized lactose retained the spherical shape as they have in vivo. Liver-specific functions such as albumin secretion and glucose metabolism were stably maintained for seven days. The metabolic activities of hepatocytes cultured on the oxidized lactose cross-linked chitosan microcarriers were higher than those of hepatocytes on chitosan microcarriers cross-linked by glutaraldehyde and on cytodex 3. The results suggest that oxidized lactose could be an interesting cross-linking agent for chitosan thus reducing the toxic side effects caused by using glutaraldehyde.
以经高碘酸钠氧化得到的氧化乳糖作为交联剂制备壳聚糖微载体。研究并优化了氧化乳糖用量对壳聚糖微载体制备的影响。在由氧化乳糖交联的壳聚糖微载体上培养的大鼠肝细胞保持了其在体内时的球形形状。白蛋白分泌和葡萄糖代谢等肝脏特异性功能稳定维持了七天。在由氧化乳糖交联的壳聚糖微载体上培养的肝细胞的代谢活性高于在由戊二醛交联的壳聚糖微载体和3号细胞培养微载体上培养的肝细胞。结果表明，氧化乳糖可能是一种用于壳聚糖的有趣的交联剂，从而减少使用戊二醛所引起的毒副作用。
