Kawase M, Michibayashi N, Nakashima Y, Kurikawa N, Yagi K, Mizoguchi T
Faculty of Education, Kagawa University, Japan.
Biol Pharm Bull. 1997 Jun;20(6):708-10. doi: 10.1248/bpb.20.708.
The effectiveness of chitosan, a biocompatible polymer derived by the deacetylation of chitin, as a scaffold of hepatocyte attachment, was examined. Since chitosan gel was too fragile to use for cell culture, its free amino groups were crosslinked by glutaraldehyde to increase its strength. Rat hepatocytes seeded onto glutaraldehyde-crosslinked chitosan (GA-chitosan) gel could stably attach to the surface, retaining its spherical form, the same as in vivo, and then release a very small amount of lactate dehydrogenase during the 5 d culture period. By contrast, hepatocytes on a collagen-coated surface spread flat, and they released much more lactate dehydrogenase than those on the GA-chitosan gel. Hepatocytes on GA-chitosan also retained higher urea synthesis activity, a liver-specific function, than those on the collagen-coated surface. These results indicate that chitosan is a promising biopolymer as a scaffold of hepatocyte attachment, which can be applied to an effective bioartificial liver support system.
研究了壳聚糖（一种由甲壳素脱乙酰化衍生而来的生物相容性聚合物）作为肝细胞附着支架的有效性。由于壳聚糖凝胶过于脆弱，无法用于细胞培养，因此其游离氨基通过戊二醛交联以增强其强度。接种到戊二醛交联壳聚糖（GA-壳聚糖）凝胶上的大鼠肝细胞能够稳定地附着在表面，保持其与体内相同的球形形态，并且在5天的培养期内释放出非常少量的乳酸脱氢酶。相比之下，胶原包被表面上的肝细胞会扁平铺展，并且它们释放的乳酸脱氢酶比GA-壳聚糖凝胶上的肝细胞多得多。GA-壳聚糖上的肝细胞也比胶原包被表面上的肝细胞保留了更高的尿素合成活性（一种肝脏特异性功能）。这些结果表明，壳聚糖作为肝细胞附着支架是一种有前途的生物聚合物，可应用于有效的生物人工肝支持系统。
