一种基于固定在明胶中的脂氧合酶的酶电极，用于选择性测定必需脂肪酸。

An enzyme electrode based on lipoxygenase immobilized in gelatin for selective determination of essential fatty acids.

作者信息

Timur Suna, Onal Seçil, Akyilmaz Erol, Telefoncu Azmi

机构信息

Department of Biochemistry, Faculty of Science, Ege University, Bornova-Izmir, Turkey.

出版信息

Artif Cells Blood Substit Immobil Biotechnol. 2003 Aug;31(3):329-37. doi: 10.1081/bio-120023162.

DOI:10.1081/bio-120023162

PMID:12906313

Abstract

An enzyme electrode for the specific determination of omega-3 and omega-6 fatty acids from the mixture of essential fatty acids (EFAs) was developed by using lipoxygenase (LOX) (EC 1.13.11.12) from soy beans in combination with a dissolved oxygen (DO) probe. The enzyme electrode showed different sensitivities for linoleic (LA) and alpha-linolenic acids (ALA), the most common essential fatty acids. Enzyme electrode response depends linearly on LA concentration between 12.8-160.5 microM and ALA concentration between 3.8-18.9 microM in borate buffer, 0.2 M at pH 9.0. However, in phosphate buffer 0.2 M at pH 6.0 linearity is in the range of 7.5-22.5 microM of ALA concentration at 5 minutes response times. Moreover, maximum electrode response was found in borate buffer at pH 9.0 and 30 degrees C.

摘要

通过将大豆中的脂氧合酶（LOX）（EC 1.13.11.12）与溶解氧（DO）探头结合使用，开发了一种用于从必需脂肪酸（EFA）混合物中特异性测定ω-3和ω-6脂肪酸的酶电极。该酶电极对亚油酸（LA）和α-亚麻酸（ALA）这两种最常见的必需脂肪酸表现出不同的灵敏度。在pH 9.0的0.2 M硼酸盐缓冲液中，酶电极响应在12.8 - 160.5微摩尔的LA浓度和3.8 - 18.9微摩尔的ALA浓度之间呈线性关系。然而，在pH 6.0的0.2 M磷酸盐缓冲液中，在5分钟响应时间下，线性范围为7.5 - 22.5微摩尔的ALA浓度。此外，在pH 9.0和30摄氏度的硼酸盐缓冲液中发现了最大电极响应。