Dinckaya E, Telefoncu A
Department of Biochemistry, Faculty of Science, Ege University, Bornova-Izmir, Türkiye.
Indian J Biochem Biophys. 1993 Oct;30(5):282-4.
A biosensor for the specific determination of oxalate was developed using oxalate oxidase (EC 1.2.3.4) from barley (Hordeum vulgare) seedling roots in combination with a dissolved oxygen probe. Oxalate oxidase immobilized with gelatin using glutaraldehyde and fixed on pretreated teflon membrane served as an enzyme electrode. The electrode response was maximum when 50 mM succinate buffer was used at pH 3.2 and 35 degrees C. The biosensor response depends linearly on oxalate concentration between 5 x 10(-6)-2 x 10(-4) M with response time 30 sec and substrate specificity of the oxalate oxidase electrode of 100%. The sensor is stable for more than 3 months during which time more than 400 assays can be performed.
利用来自大麦（Hordeum vulgare）幼苗根的草酸氧化酶（EC 1.2.3.4）与溶解氧探头相结合，开发了一种用于特异性测定草酸盐的生物传感器。用戊二醛将草酸氧化酶与明胶固定，并固定在预处理的聚四氟乙烯膜上，用作酶电极。当在pH 3.2和35℃下使用50 mM琥珀酸盐缓冲液时，电极响应最大。生物传感器响应在5×10(-6)-2×10(-4) M的草酸盐浓度之间呈线性关系，响应时间为30秒，草酸氧化酶电极的底物特异性为100%。该传感器在3个月以上的时间内稳定，在此期间可进行400多次测定。
