Oliveira A C, Peres C M, Correia Pires J M, Silva Pereira C, Vitorino S, Figueiredo Marques J J, Barreto Crespo M T, San Romão M V
Instituto de Biologia Experimental e Tecnológica/Instituto de Tecnologia Química e Biológica-Universidade Nova de Lisboa, Apt. 12,2781-901 OEIRAS, Portugal.
Microbiol Res. 2003;158(2):117-24. doi: 10.1078/0944-5013-00183.
The main aims of this work were the study of cork slabs moulds colonization and the evaluation of the moulds diversity during cork processing steps, in different cork stoppers factories. Simultaneously, it was envisaged to perform an evaluation of the air quality.
Moulds were isolated and identified from cork slabs and cork samples in four cork stoppers factories. The identification was based on morphological characters and microscopic observation of the reproductive structures. Airborne spore dispersion was assessed using a two stage Andersen sampler. It was observed that Chrysonilia sitophila was always present on cork slabs during the maturing period, but mould diversity appeared to be associated to the different factory configurations and processing steps.
Spatial separation of the different steps of the process, including physical separation of the maturation step, is essential to guarantee high air quality and appropriate cork slabs colonization, i.e. C. sitophila dominance. The sorting and cutting of the edges of cork slabs after boiling and before the maturing step is also recommended.
This study is very important for the cork stopper industry as it gives clear indications on how to keep high quality manufacturing standards and how to avoid occupational health problems.
本研究的主要目的是研究软木塞模具的定殖情况，并评估不同软木塞工厂软木加工步骤中霉菌的多样性。同时，还设想对空气质量进行评估。
从四个软木塞工厂的软木板和软木样品中分离并鉴定霉菌。鉴定基于形态特征和生殖结构的显微镜观察。使用两级安德森采样器评估空气中孢子的扩散情况。观察到在成熟期间嗜卷书虱始终存在于软木板上，但霉菌多样性似乎与不同的工厂配置和加工步骤有关。
对加工过程的不同步骤进行空间隔离，包括对成熟步骤进行物理隔离，对于保证高质量的空气质量和软木板的适当定殖（即嗜卷书虱占优势）至关重要。还建议在煮沸后和成熟步骤之前对软木板的边缘进行分选和切割。
本研究对软木塞行业非常重要，因为它明确指出了如何保持高质量的制造标准以及如何避免职业健康问题。
