Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras, Portugal.
FEMS Microbiol Ecol. 2012 Oct;82(1):202-14. doi: 10.1111/j.1574-6941.2012.01419.x. Epub 2012 Jun 27.
A particular fungal population is present in the main stages of the manufacturing process of cork discs. Its diversity was studied using both dependent (isolation) and independent culture methods (denaturing gel gradient electrophoresis and cloning of the ITS1-5.8S-ITS2 region). The mycobiota in the samples taken in the stages before and after the first boiling seems to be distinct from the population in the subsequent manufacturing stages. Most isolated fungi belong to the genera Penicillium, Eurotium and Cladosporium. The presence of uncultivable fungi, Ascomycota and endophytes in raw cork was confirmed by sequencing. The samples taken after the first boiling contained uncultivable fungi, but in a few samples some isolated fungi were also detected. The main taxa present in the following stages were Chrysonilia sitophila, Penicillium glabrum and Penicillium spp. All applied techniques had complementary outcomes. The main factors driving the shift in cork fungal colonization seem to be the high levels of humidity and temperature to which the slabs are subjected during the boiling process.
特定的真菌种群存在于软木圆盘制造过程的主要阶段。使用依赖（分离）和独立培养方法（变性凝胶梯度电泳和 ITS1-5.8S-ITS2 区域的克隆）研究了其多样性。在第一次煮沸前后取样的样本中的真菌区系似乎与后续制造阶段的种群不同。大多数分离出的真菌属于青霉属、曲霉属和枝孢属。通过测序证实了未培养真菌、子囊菌和内生菌在天然软木中的存在。第一次煮沸后的样本中含有不可培养的真菌，但在少数样本中也检测到了一些分离出的真菌。在随后的阶段中主要存在的分类群是 Chrysonilia sitophila、Penicillium glabrum 和 Penicillium spp。所有应用的技术都有互补的结果。导致软木真菌定殖转移的主要因素似乎是板在煮沸过程中所经历的高湿度和高温。
